FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005407866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4156744
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTCGTAT446671.0745670168766708TruSeq Adapter, Index 22 (97% over 37bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA179700.4323095191813593No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCGCGTAT171110.4116443062165964TruSeq Adapter, Index 22 (97% over 37bp)
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT160060.3850609996670471No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC143000.3440192612294623Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC136540.3284782512466488Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG135810.3267220690040089No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG95890.23068536335170026TruSeq Adapter, Index 2 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTCGTTT94110.22640316555457832TruSeq Adapter, Index 22 (97% over 37bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG79520.19130357799277511Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTA65570.15774365705465623TruSeq Adapter, Index 22 (97% over 37bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC63600.153004370728628Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA62610.15062269892011634No Hit
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA58280.1402058919192522Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAG57450.13820913676666158No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT56320.13549066288421902Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCTCGGAT53280.12817724642171854TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTATAGGATCGCGGAT45430.1092922729905907TruSeq Adapter, Index 22 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)