FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005407867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4156744
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG339460.8166488001185542No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG137460.330691522018195Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC133490.3211407774931533Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTG92910.22351629063517023Illumina Single End PCR Primer 1 (96% over 33bp)
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG81140.19520085913397603No Hit
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG74740.17980419289713295Illumina Single End PCR Primer 1 (100% over 30bp)
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG73910.17780743774454236No Hit
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGGA64480.15512141233619392Illumina Single End PCR Primer 1 (96% over 33bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA64300.1546883810982827No Hit
ATACGACTCACTATAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGA60780.14622021466801902No Hit
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG57590.13854593884059255Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA47450.1141518457715943Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG43500.10464921582854272Illumina Single End PCR Primer 1 (100% over 24bp)
TTGTACTCTAGTTGTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC41570.10000615866649473No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)