FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005407876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49231103
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2395190.48651967029867277Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1774630.3604692748809629Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC1358330.2759089106738072No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG884190.17959987611896486TruSeq Adapter, Index 2 (97% over 35bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCG848900.1724316434673422TruSeq Adapter, Index 1 (97% over 36bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC833320.169266977422789No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT819530.16646590266320055No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT662310.13453080667317163No Hit
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG657670.13358831306298377Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC592770.12040558993772696No Hit
CTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTA589560.11975356310826513No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGAG541200.10993050470553137TruSeq Adapter, Index 1 (97% over 36bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC535270.10872598162182147Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG515290.10466757163657292No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)