FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005407878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5560043
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC313980.5647078628708447Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC232380.4179464079684276Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC154630.27810935994559755No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG118470.21307389169472252TruSeq Adapter, Index 2 (97% over 35bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCG113020.20327180922881352TruSeq Adapter, Index 1 (97% over 36bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC95970.17260657876207072No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT91730.16498073845831768No Hit
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG86270.15516067051999416Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT74330.13368601645706696No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGAG74240.13352414720533637TruSeq Adapter, Index 1 (97% over 36bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC69810.1255565829257076Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTA69710.12537672820156245No Hit
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC67810.12195948844280521No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG58390.105017173428335No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)