FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005407914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7361127
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1973742.6813013822475824Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1947652.6458584398829146Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG1769372.403667264537074Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1587012.1559334596455133Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC1428781.9409799613564607Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA1411381.917342276529124Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT1389201.887211020812438Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1246051.6927435160404107No Hit
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG1172061.5922290160188788Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1029821.3989977349935683Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCTCGTAT897301.2189709537683564TruSeq Adapter, Index 11 (97% over 39bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG815721.108145532606624Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC755161.0258755215064215Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG734610.9979586006327564TruSeq Adapter, Index 2 (97% over 35bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC697080.9469745597379314No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT672220.9132025571627823Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA649130.8818350776993795Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA635270.8630064390955353No Hit
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT568290.7720149373866257Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCTCGTTT453100.6155307468543879TruSeq Adapter, Index 11 (97% over 39bp)
TATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG399940.5433135442439724TruSeq Adapter, Index 2 (97% over 35bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG396380.5384773282678047Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCGCGTAT345600.46949332622572604TruSeq Adapter, Index 11 (97% over 39bp)
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATCGAGATCGGAAGAGCA329610.44777110896198363No Hit
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCT327800.44531224634488714TruSeq Adapter, Index 11 (97% over 37bp)
TATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAAGATCGGAAGAGCACA316500.42996133608345577No Hit
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCACACG306540.416430799251256No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG279160.3792354078390442No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCTCGGAT270230.3671041132696121TruSeq Adapter, Index 11 (97% over 39bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC259210.35213357954563207No Hit
AAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATTA250840.34076303805110275No Hit
TATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCT229120.3112566866459443TruSeq Adapter, Index 11 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCTCGGTT225630.30651556480413933TruSeq Adapter, Index 11 (97% over 39bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTAC174700.23732778961699752TruSeq Adapter, Index 11 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCGCGTTT153280.2082289845019655TruSeq Adapter, Index 11 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCGCGGAT146750.19935806025354544TruSeq Adapter, Index 11 (97% over 39bp)
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG142120.1930682625092598No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCTCGTGT142070.19300033812757203TruSeq Adapter, Index 11 (97% over 39bp)
ATATATATATATATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAG133720.18165696638571782Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC127980.17385924736796418No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCGCGGTT126410.17172642178296885TruSeq Adapter, Index 11 (97% over 39bp)
ATATATATATATATATATATGTAGATCGGAAGAGCACACGTCTGAACTCC120650.16390153301254007Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATATGTAGATCGGAAGAGCACACGTCTGAACT119380.16217625371767122Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATATGTAGATCGGAAGAGCACACGTCTGAA118380.1608177660839162Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC117820.16005701300901343Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGAGAGAGATCGGAAGAGCA114460.15549249455959663No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCTCGGGT112670.1530608016951752TruSeq Adapter, Index 11 (97% over 39bp)
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGC111310.15121325851326842No Hit
ATATATATATATATATATATATATATGTAGATCGGAAGAGCACACGTCTG107010.14537176168812194Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGAGCGAGATCGGAAGAGCA95630.1299121724159901No Hit
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCACACG81550.11078466653271979No Hit
ATATATATATATATATATATATATATATGTAGATCGGAAGAGCACACGTC80720.10965712179670313No Hit
ATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGATCGGAAGAGCACACG76640.10411449225098275No Hit
TATATATATATATATATATATATATATATATATAAGATCGGAAGAGCACA75650.10276958949356532No Hit
TATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTAC75280.10226694906907596TruSeq Adapter, Index 11 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCGCGGGT74850.10168279938656132TruSeq Adapter, Index 11 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)