FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005407915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7361127
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1935522.6293799848854666Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC1913332.5992351442924435Illumina Single End PCR Primer 1 (96% over 33bp)
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG1711992.3257172441122127No Hit
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1708432.320881028136045Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1555992.1137931732464335Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG1344891.8270164337607544Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA1272131.7281728735287412Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG1263311.7161910125990216Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC1002161.3614219670439052Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG994611.3511653854090548Illumina Single End PCR Primer 1 (100% over 22bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG781251.0613184638710893Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTT720090.9782333601906338Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG700270.9513081352896099Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA596020.8096857994706518Illumina Single End PCR Primer 1 (100% over 26bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT585120.7948782842627223Illumina Single End PCR Primer 1 (100% over 31bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT574810.7808722767587083No Hit
TATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGT557660.75757421383981No Hit
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG551870.7497085704403686Illumina Single End PCR Primer 1 (100% over 24bp)
AAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATTA520750.7074324352779133No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG496450.674421185777667No Hit
TATCCCCTGAGACTGCCAAGGCACACAGGGGATAAGATCGGAAGAGCGTC453170.6156258409887508No Hit
TATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTT385980.5243490568767527Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG310840.4222722960764025Illumina Single End PCR Primer 1 (100% over 22bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTAG300700.4084972314701268Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGT238540.32405364015591637No Hit
TCCAGCATTAGAGGATACATAGCAGATAGTACCAAACCCAGTGTCTATGT218940.29742728253431844No Hit
TATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTAG207120.2813699587033344Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT189450.25736548221488365No Hit
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTAGGA160190.21761613405121252Illumina Single End PCR Primer 1 (96% over 33bp)
ACATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG130550.17735056058671453Illumina Single End PCR Primer 1 (100% over 30bp)
ACATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG115610.1570547553384149Illumina Single End PCR Primer 1 (100% over 32bp)
ACATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG115030.156266832510837Illumina Single End PCR Primer 1 (100% over 28bp)
CAAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATT112290.15254457639434832No Hit
ACATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA109760.14910760268094816Illumina Single End PCR Primer 1 (100% over 26bp)
ACATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG105670.14355138825889027Illumina Single End PCR Primer 1 (100% over 24bp)
ACATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG92450.12559218174064923Illumina Single End PCR Primer 1 (100% over 22bp)
ATCCCCTGAGACTGCCAAGGCACACAGGGGATAGATCGGAAGAGCGTCGT90480.12291596110215189No Hit
CCAGCATTAGAGGATACATAGCAGATAGTACCAAACCCAGTGTCTATGTA77850.10575826228782631No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGG76180.10348958793945547No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)