FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005407916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42900996
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC12223992.8493487657023158Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC12010572.799601668921626Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG11020332.56878185299008Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCTCGTAT10213442.3806999725600777TruSeq Adapter, Index 11 (97% over 39bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC9907952.309491835574167Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC8857652.06467234467004Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA8645682.015263235380363Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT8575371.9988743384885517Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA8495451.980245400363199No Hit
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG7127771.6614462750468544Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT6858791.5987484299898305No Hit
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC6409201.4939513292418665Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG4997531.1648983627326508Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC4596521.0714250084077301Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG4534891.0570593745655694TruSeq Adapter, Index 2 (97% over 35bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC4175130.9732011816229161No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT4117690.9598122150823724Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA3895230.9079579411163321Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT3736450.8709471453762986Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATCGAGATCGGAAGAGCA3136780.731167173834379No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCGCGTAT3115670.7262465421548722TruSeq Adapter, Index 11 (97% over 39bp)
TATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAAGATCGGAAGAGCACA3074560.7166640140475993No Hit
TATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG2516100.5864898800950915TruSeq Adapter, Index 2 (97% over 35bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG2375750.5537750219132441Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCTCGTTT2242220.522649870413265TruSeq Adapter, Index 11 (97% over 39bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCT2016420.47001705974378777TruSeq Adapter, Index 11 (97% over 37bp)
TCCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATG1872840.4365493052888562No Hit
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCACACG1825830.4255915177353924No Hit
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGAGAGAGATCGGAAGAGCA1638280.3818745839840175No Hit
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC1501900.35008511224308175No Hit
TATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCT1454760.33909702236283745TruSeq Adapter, Index 11 (97% over 37bp)
AAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATTA1343780.3132281590851643No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCTCGGAT1093150.25480760400061575TruSeq Adapter, Index 11 (97% over 39bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTAC1071060.24965853939614827TruSeq Adapter, Index 11 (97% over 39bp)
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGAGCGAGATCGGAAGAGCA883130.20585302961264582No Hit
ATATATATATATATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAG805960.18786510224611103Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCGCGGAT778530.18147131129543007TruSeq Adapter, Index 11 (97% over 39bp)
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG769440.17935247936901047No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCGCGTTT746160.1739260319270909TruSeq Adapter, Index 11 (97% over 39bp)
ATATATATATATATATATATGTAGATCGGAAGAGCACACGTCTGAACTCC742900.17316614280936507Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC718840.16755788140676267Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATATATATGTAGATCGGAAGAGCACACGTCTGAACT718260.16742268641035749Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATATGTAGATCGGAAGAGCACACGTCTGAA702530.16375610486991957Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC679840.15846718337261914No Hit
CCAGCATTAGAGGTAACAACTAGAGTACAACAGCCACTAGTAGCCTATGC655160.152714403180756No Hit
ATATATATATATATATATATATATATGTAGATCGGAAGAGCACACGTCTG632080.14733457470311412Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGATCGGAAGAGCACACG529670.12346333404473872No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCTCGGTT487300.11358710646251662TruSeq Adapter, Index 11 (97% over 39bp)
TATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTAC474640.11063612602374079TruSeq Adapter, Index 11 (97% over 39bp)
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCACACG468420.1091862762347056No Hit
ATATATATATATATATATATATATATATGTAGATCGGAAGAGCACACGTC464250.1082142708295164No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)