FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005407917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42900996
Sequences flagged as poor quality0
Sequence length151
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG17339244.041687050808797No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG11552522.692832585984717Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC11498242.6801801990797602Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG10215932.3812803786653345Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG9392542.1893524336824255Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG7973291.8585326084270863Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA7356091.71466648466623Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG7027711.6381228072187413Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC6115851.42557296338761Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG5311091.237987574927165Illumina Single End PCR Primer 1 (100% over 22bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG4580211.0676232318708871Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTT4338521.01128654448955Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG4025360.9382905702235911Illumina Single End PCR Primer 1 (100% over 28bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT3623380.8445911139219239Illumina Single End PCR Primer 1 (100% over 31bp)
TATCCCCTGAGACTGCCAAGGCACACAGGGGATAAGATCGGAAGAGCGTC3603520.8399618507691523No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3493370.8142864561932315No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA3353860.7817673976613503Illumina Single End PCR Primer 1 (100% over 26bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT3009970.7016084195341292No Hit
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG2919030.6804107764770776Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGT2883300.6720822985088738No Hit
AAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATTA2817130.6566584141776103No Hit
TATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTT2383410.5555605282450785Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTAG1813390.42269181815732204Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG1552460.361870386412474Illumina Single End PCR Primer 1 (100% over 22bp)
TCCAGCATTAGAGGATACATAGCAGATAGTACCAAACCCAGTGTCTATGT1332360.31056621622491004No Hit
TATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTAG1300070.30303958444228196Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGT1241670.28942684687320547No Hit
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTAGGA932600.2173842304267248Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT898310.20939140900132014No Hit
ACATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG762580.17775344889428674Illumina Single End PCR Primer 1 (100% over 30bp)
ACATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG688860.16056969866154155Illumina Single End PCR Primer 1 (100% over 32bp)
ACATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG683150.15923872723141438Illumina Single End PCR Primer 1 (100% over 28bp)
ACATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA627470.14626000757651408Illumina Single End PCR Primer 1 (100% over 26bp)
CAAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATT599190.13966808602765304No Hit
ACATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG579950.13518334166414225Illumina Single End PCR Primer 1 (100% over 24bp)
ATCCCCTGAGACTGCCAAGGCACACAGGGGATAGATCGGAAGAGCGTCGT567690.13232559915392175No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGG544810.12699238964055753No Hit
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGGAGGG542900.12654717853170588Illumina Single End PCR Primer 1 (95% over 24bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGGAG509500.11876181149733679Illumina Single End PCR Primer 1 (95% over 22bp)
ACATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG477180.11122818687006708Illumina Single End PCR Primer 1 (100% over 22bp)
CCAGCATTAGAGGATACATAGCAGATAGTACCAAACCCAGTGTCTATGTA442230.10308152286254613No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)