FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005407927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53102195
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG1175140.22129782017485342No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC846060.1593267472276805No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG834080.15707071995799796Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG748780.1410073538391398Illumina Single End PCR Primer 1 (96% over 33bp)
CGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCG542910.10223871160128127No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)