FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005407938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14870252
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT384020.2582471366322508No Hit
GCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAA317000.21317728845482914No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA281160.18907547767179736No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCACTGATCTCGTAT267570.17993642609419128TruSeq Adapter, Index 27 (97% over 37bp)
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG257200.1729627715791232No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA221900.14922410191838043No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC194930.13108722031072506No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG193420.1300717701354355No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC179150.12047542973716921Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGC175320.11789981770315662No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC167320.11251994922480131Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG155070.10428202561731974No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)