FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005407946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55271369
Sequences flagged as poor quality0
Sequence length151
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC5729161.036551130115847No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG3809010.6891470337924867No Hit
CGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGA1550300.2804888006302142No Hit
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCGGGTGCCCGGGCCCCCCTCG1281060.23177641936098958No Hit
CCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCG1118390.20234526848792184No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1113270.20141892993459234No Hit
CTCCCCGGCGCGGCGGGCGAGACGGGCCGGTGGTGCGCCCTCGGCGGACT1031970.18670968689051287No Hit
CCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACC942460.17051504550212968No Hit
CTCGTCTCACTCAAACCGCCTCGAACCCCACACCGACGAGCTCCCTCAGG919530.1663664238170037No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC869420.1573002470772888No Hit
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG867490.15695106086480326Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCGACTCCGGGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCC831960.150522777896093No Hit
CTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCGGGTGCCCGGGCCCC805270.14569387633586567No Hit
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC790180.14296371056052548Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC780570.1412250165180457No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC762710.13799368711131438No Hit
CTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCGATCCACGGGA743980.13460495252071647No Hit
GGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGC713760.12913738395008814No Hit
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC685860.12408956253643726Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCT619280.11204354283318005No Hit
GTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGT595790.10779360286878367No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAAGGTTATCTCGTAT589630.10667910179680913TruSeq Adapter, Index 2 (97% over 36bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC588880.10654340767278625Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC573820.10381866966240696Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGC563570.10196418330076101No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG553530.10014769129384148No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)