FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005407948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12834706
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG2211371.722961164829175Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC2177211.6963458298148784Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACGGTTATCTCGTAT1977421.5406819603035706TruSeq Adapter, Index 9 (97% over 37bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG1751581.3647215604315361Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC1672791.3033333213865592Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA1671811.302569766693526Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT1514241.179801079977991Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1441411.1230565000865622Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1439341.1214436855818903Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1383001.077547082106906Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC1370841.0680727708137607No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACGGTTATCGCGTAT1083130.8439071374131981TruSeq Adapter, Index 9 (97% over 37bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG1041860.8117521351872026Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCACACG973640.7585993789027968No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA939590.7320697490071062TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA914810.7127627231975552Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC867080.675574493097076Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT844010.6575997923131235Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG809140.6304312697150991Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATATATATATATATATATATAAGATCGGAAGAGCACA677820.5281149408486645No Hit
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC666180.519045781025292No Hit
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA656890.5118075941903149No Hit
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAC631870.4923135753947149TruSeq Adapter, Index 9 (97% over 37bp)
TATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA622970.48537925216206745TruSeq Adapter, Index 7 (97% over 35bp)
TATATATATATATATATATATATATATATATATATAAGATCGGAAGAGCA466430.36341307701165887No Hit
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCACACG466140.3631871271535164No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT417150.3250171838762804No Hit
TATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAC416800.3247444857716258TruSeq Adapter, Index 9 (97% over 37bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACGG377060.29378156383169196TruSeq Adapter, Index 9 (97% over 37bp)
ATATATATATATATATATATATATATATATATATAGATCGGAAGAGCACA345050.26884137431741717No Hit
TATATATATATATATATATATATATATATATATATATAAGATCGGAAGAG252710.19689582293509492No Hit
ATATATATATATATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAG197730.15405884638105463Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATATATATATATATATAGATCGGAAGAGCA197160.15361473803918843No Hit
ATATATATATATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC186510.14531692428326756Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACGGTT176380.13742426199711938TruSeq Adapter, Index 9 (97% over 37bp)
ATATATATATATATATATATGTAGATCGGAAGAGCACACGTCTGAACTCC169460.1320326308993755Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATATGTAGATCGGAAGAGCACACGTCTGAACT158660.12361794652717406Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATATGTAGATCGGAAGAGCACACGTCTGAA156920.12226224737831937Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACGG156620.12202850614575823TruSeq Adapter, Index 9 (97% over 37bp)
ATATATATATATATATATATATATATGTAGATCGGAAGAGCACACGTCTG155720.12132728244807478Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATATATATATATATATATATATATGTAGATCGGAAGAGCACACGTC141580.11031027902002587No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)