FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005407949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12834706
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG2226831.735006629680493Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG2192151.7079861431964236Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG1730361.3481882639150442Illumina Single End PCR Primer 1 (100% over 22bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG1685161.3129712515424974Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG1673011.3035047316237707Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA1501581.1699371999639103Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1464671.14117923698447Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA1445991.126624949570329Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA1407511.096643740807152Illumina Single End PCR Primer 1 (100% over 34bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGT1332561.038247389538958No Hit
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1060360.8261661778618069Illumina Single End PCR Primer 1 (100% over 30bp)
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG986510.7686268777796702No Hit
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATT951720.7415206861769954Illumina Single End PCR Primer 1 (97% over 34bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG900000.7012236976834529Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGT898810.7002965241276271No Hit
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG876710.683077586662289Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA832040.6482735171339336Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG775450.6041821292984818Illumina Single End PCR Primer 1 (100% over 22bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATTCC628170.48943076685979403Illumina Single End PCR Primer 1 (97% over 34bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT623400.4857142812620717No Hit
TATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATT621030.4838677255248387Illumina Single End PCR Primer 1 (97% over 34bp)
TATATATATATATATATATATATATATATATATAAGATCGGAAGAGCGTC584290.45524221591051633No Hit
TATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATTCC406070.3163843410203553Illumina Single End PCR Primer 1 (97% over 34bp)
TATATATATATATATATATATATATATATATATATAAGATCGGAAGAGCG402750.31379760471334517No Hit
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGT402380.31350932385985314No Hit
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATTCCTC376420.2932829158688948Illumina Single End PCR Primer 1 (97% over 34bp)
ATATATATATATATATATATATATATATATATATAGATCGGAAGAGCGTC266480.20762454550965173No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG238100.18551262490936685No Hit
TATATATATATATATATATATATATATATATATATATAAGATCGGAAGAG224540.17494752119760282No Hit
AAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATTA222200.17312433958362586No Hit
ACATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG199400.15536000590897836Illumina Single End PCR Primer 1 (100% over 30bp)
ACATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG187910.14640771670188626Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATTCCTCCG177550.13833585280410787Illumina Single End PCR Primer 1 (97% over 34bp)
ACATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG169360.13195471715518844Illumina Single End PCR Primer 1 (100% over 28bp)
ACATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA159180.1240230979969467Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATATATATATATATATATATATATAGATCGGAAGAGCG156330.12180255628761577No Hit
ACATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG155790.12138182206900572Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATTCCTC155200.12092213097830212Illumina Single End PCR Primer 1 (97% over 34bp)
ACATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG152880.11911453211316254Illumina Single End PCR Primer 1 (100% over 22bp)
ACATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT137020.10675741228509637No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)