FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005407951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23922437
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG4409441.8432235812764393Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG4386401.833592455484364Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG3336381.3946656020036756Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG3244471.3562456032384995Illumina Single End PCR Primer 1 (100% over 22bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG3237861.3534825068198528Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA2948031.2323284621880286Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA2917471.2195538439499287Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG2898061.21144012209124Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA2791071.1667164177295148Illumina Single End PCR Primer 1 (100% over 34bp)
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG2736991.1441100252453378No Hit
TATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGT2432921.0170034098114669No Hit
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG2082330.8704506150439439Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATT1900680.7945177157327241Illumina Single End PCR Primer 1 (97% over 34bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG1723710.7205411388480195Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG1690420.7066253325277856Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGT1603910.6704626288701272No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA1603160.6701491156607499Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG1401830.5859896297354654Illumina Single End PCR Primer 1 (100% over 22bp)
TATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATT1272810.532056997370293Illumina Single End PCR Primer 1 (97% over 34bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATTCC1261320.5272539750026304Illumina Single End PCR Primer 1 (97% over 34bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT1083440.4528970020905479No Hit
TATATATATATATATATATATATATATATATATAAGATCGGAAGAGCGTC1021250.42690048676896925No Hit
TATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATTCC841500.3517618209215056Illumina Single End PCR Primer 1 (97% over 34bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATTCCTC759760.3175930612754879Illumina Single End PCR Primer 1 (97% over 34bp)
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGT691600.28910098080726476No Hit
TATATATATATATATATATATATATATATATATATAAGATCGGAAGAGCG677160.2830648064827175No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG632730.264492283959197No Hit
ATATATATATATATATATATATATATATATATATAGATCGGAAGAGCGTC437590.18292032705530795No Hit
AAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATTA402800.16837749431631902No Hit
ACATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG384480.16071941165525905Illumina Single End PCR Primer 1 (100% over 30bp)
ACATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG370400.15483372367121295Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATATATATATATATATATATAAGATCGGAAGAG361550.15113426780055894No Hit
ATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATTCCTCCG357190.14931171101004467Illumina Single End PCR Primer 1 (97% over 34bp)
ACATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG331630.13862718083446096Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATTCCTC319520.13356498754704632Illumina Single End PCR Primer 1 (97% over 34bp)
ACATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA302680.12652557095249117Illumina Single End PCR Primer 1 (100% over 26bp)
ACATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG289970.12121256709757455Illumina Single End PCR Primer 1 (100% over 24bp)
ACATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG283680.11858323631492894Illumina Single End PCR Primer 1 (100% over 22bp)
TATCCCCTGAGACTGCCAAGGCACACAGGGGATAAGATCGGAAGAGCGTC268560.11226281001387944No Hit
ATATATATATATATATATATATATATATATATATATAGATCGGAAGAGCG246980.10324198993605876No Hit
ACATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT245040.10243103576780242No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)