FastQCFastQC Report
Sun 5 Jun 2022
EGAF00005407959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28121476
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT661360.23517968971472195No Hit
CTTGAAGCCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCT385490.13708028696644514No Hit
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA350090.12449204302078597Illumina Single End PCR Primer 1 (100% over 34bp)
CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGA333170.11847528913489463No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG329830.11728758476262056No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG321570.11435032784196676Illumina Single End PCR Primer 1 (100% over 32bp)
CTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGA319220.1135146675800374No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG294950.10488425287492022No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)