FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005407960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44407906
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT964000.21707846346098822No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG905470.20389837791495954No Hit
CTCCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCA819950.18464054576227937No Hit
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC813110.18310027948626986No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG779020.17542371847031024No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC714930.16099160361220363No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC704520.15864742642897864Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG691540.15572452346660975No Hit
CGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGC675600.152135072525149No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC670710.15103391724887907No Hit
CGCTCCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAA666100.14999581380846916No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG665560.14987421383931052No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC652000.1468207035026601Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA645280.14530745944201917No Hit
CTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTA608790.13709045411868778No Hit
GTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGAC604650.13615818768847152No Hit
CACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCT583050.13129418892212572No Hit
CTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGG569790.12830823412389677No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC569520.12824743413931744No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA562760.12672518267355368No Hit
CTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATG544840.12268986517851123No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG528850.11908915498064691No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC513760.11569111139804701No Hit
CTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCA505410.11381081557864944No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT462440.10413461062541432No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG462050.10404678842546641No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA446140.1004640930378478TruSeq Adapter, Index 7 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)