FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005407970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8738091
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3407643.8997533900711265No Hit
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG2178602.4932219176934645Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1979882.2658038237413645Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCATTAATCTCGTAT1806942.067888741373831TruSeq Adapter, Index 7 (97% over 36bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC1644351.8818183513996365Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1606921.8389829082805387No Hit
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT1256601.4380715421709387Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1189161.3608922131847792Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA1070601.2252104035080431Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG940291.076081720824377Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC889631.0181056709068377Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC878831.0057459918877016Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC708540.810863608538753No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCATTAATCGCGTAT616840.7059207783484973TruSeq Adapter, Index 7 (97% over 36bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG596090.6821741728256207Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG536500.6139784994228143TruSeq Adapter, Index 2 (100% over 35bp)
TATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG524090.5997763126980481TruSeq Adapter, Index 2 (100% over 35bp)
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCACACG508420.5818433339730611No Hit
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC428280.49012993799217697Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT421530.48240513860521705No Hit
TATATATATATATATATATATATATATATATATAAGATCGGAAGAGCACA369700.42309012346060487No Hit
TATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGC349600.40008738750832423TruSeq Adapter, Index 7 (97% over 36bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT338940.3878879265505475Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGC333320.3814563158017008TruSeq Adapter, Index 7 (97% over 36bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA328440.3758715719486099Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATATATATATATATATATATATAAGATCGGAAGAGCA294200.33668681179905313No Hit
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG265480.30381922092594366Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC232640.26623664138997866No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCAT195730.22399629392735781TruSeq Adapter, Index 7 (97% over 36bp)
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA190410.2179080075957094No Hit
TATATATATATATATATATATATATATATATATATATAAGATCGGAAGAG183960.21052653262594773No Hit
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCACACG180050.2060518710551309No Hit
CCCAGACCCGCCGCGACGCCGCCGAGAACCGCCCCCGCGCCCGCCGACAC178890.2047243499752978No Hit
ATATATATATATATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAG154770.177121066832561Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATATATATATATATAGATCGGAAGAGCACA149580.17118155441503183No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA148870.17036901996099607No Hit
ATATATATATATATATATATGTAGATCGGAAGAGCACACGTCTGAACTCC136670.15640716032826849Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCCCGC135900.15552596099079305No Hit
CGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGCGGGGCCGGA128380.14691996226635773No Hit
TATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCAT124990.14304039635201785TruSeq Adapter, Index 7 (97% over 36bp)
GGCGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCCCG124360.1423194150759016No Hit
ATATATATATATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC122970.14072867860954985Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCCCGCC116680.1335303099956272No Hit
ATATATATATATATATATATATGTAGATCGGAAGAGCACACGTCTGAACT115330.1319853501182352Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATATATATATATATATAGATCGGAAGAGCA103010.11788616071862837No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC96170.11005836400650897No Hit
ATATATATATATATATATATATATGTAGATCGGAAGAGCACACGTCTGAA90590.10367252984662209Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)