FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005407971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8738091
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2513152.876085863605678No Hit
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG2029232.322280690370471Illumina Single End PCR Primer 1 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1888092.160757996225949No Hit
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1874632.145354174040989Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG1505031.7223784920527836Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA1117351.2787117918547655Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT1110851.2712730961488041Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG934851.069856104725849Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG832920.9532059119091343Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT826670.9460533198841716Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG772010.883499611070656Illumina Single End PCR Primer 1 (100% over 22bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGT553880.6338684273258313No Hit
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG546200.6250793222455568Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGA501730.5741871994695409Illumina Single End PCR Primer 1 (97% over 36bp)
TATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGA484610.5545948193947625Illumina Single End PCR Primer 1 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG462620.5294291396141331No Hit
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGT383090.43841383661488537No Hit
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG382960.4382650627007661Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGACT315200.3607195210029284Illumina Single End PCR Primer 1 (97% over 36bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGACT303730.34759308411871653Illumina Single End PCR Primer 1 (97% over 36bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA290060.3319489348417177Illumina Single End PCR Primer 1 (100% over 26bp)
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG285060.32622686122174743No Hit
TATATATATATATATATATATATATATATATATAAGATCGGAAGAGCGTC277470.31754075346663246No Hit
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG272190.31149824372394386Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATATATATATATATATATATAAGATCGGAAGAGCG240270.27496852573405334No Hit
AAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATTA237050.27128351032279246No Hit
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG200310.2292377133632506Illumina Single End PCR Primer 1 (100% over 22bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGACTGA175880.20127966165607566Illumina Single End PCR Primer 1 (97% over 36bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT165760.18969818464925578No Hit
TATATATATATATATATATATATATATATATATATATAAGATCGGAAGAG151340.1731957243292614No Hit
ACATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG143220.16390307677042962Illumina Single End PCR Primer 1 (100% over 30bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT136640.1563728278865487No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA135150.15466764994779753No Hit
ACATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG124060.14197609065870337Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGT119720.13700933075656915No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117390.134342844449663No Hit
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA115540.13222567721027395No Hit
ACATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG114730.13129870128383878Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGACTGA112860.12915864574996988Illumina Single End PCR Primer 1 (97% over 36bp)
GGGGCGTGTTGCGTGCGGTGTGGTGGTGGGGGAGGAGGAAGGCGGGTCCG111320.127396247075019No Hit
GTTGCGTGCGGTGTGGTGGTGGGGGAGGAGGAAGGCGGGTCCGGAAGGGG109140.12490142297671195No Hit
GGCGTGTTGCGTGCGGTGTGGTGGTGGGGGAGGAGGAAGGCGGGTCCGGA106640.1220403861667268No Hit
ACATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA102640.11746272727075056Illumina Single End PCR Primer 1 (100% over 26bp)
GGGCGTGTTGCGTGCGGTGTGGTGGTGGGGGAGGAGGAAGGCGGGTCCGG98990.11328561352817222No Hit
GGGGGAGCCGCGGGGATCGCCGAGGGCCGGTCGGCCGCCCCGGGTGCCGC98420.11263329713549562No Hit
CGCGGGGATCGCCGAGGGCCGGTCGGCCGCCCCGGGTGCCGCGCGGTGCC96760.11073356869366548No Hit
ATATATATATATATATATATATATATATATATATAGATCGGAAGAGCGTC96220.11011558474270867No Hit
GGGGGCGTGTTGCGTGCGGTGTGGTGGTGGGGGAGGAGGAAGGCGGGTCC95750.10957770982243145No Hit
GGGAGGAGGAAGGCGGGTCCGGAAGGGGAAGGGTGCCGGCGGGGAGAGAG93070.1065106783621274No Hit
CCCCTGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91690.10493138604301558No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)