FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005407972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36196518
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11071663.058763829161689No Hit
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG9767872.6985661991023555Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC8899362.4586232300024Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCATTAATCTCGTAT8662342.3931417933625547TruSeq Adapter, Index 7 (97% over 36bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC7382632.0395967369015993Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT5602031.5476709665830288Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5379211.4861125592246194Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5201821.437105082870126No Hit
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA4760271.3151182110942274Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG4137311.1430132588996544Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC3983311.100467730072821Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3904951.078819238911323Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC3047960.8420588963833483No Hit
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG2630390.7266969712390567Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCATTAATCGCGTAT2611620.7215113895762018TruSeq Adapter, Index 7 (97% over 36bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG2408800.6654783755719266TruSeq Adapter, Index 2 (100% over 35bp)
TATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG2366170.6537009996375895TruSeq Adapter, Index 2 (100% over 35bp)
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCACACG2128700.5880952416472767No Hit
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC1885240.5208346283474007Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1716550.47423069810195556No Hit
TATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGC1601800.44252875373261036TruSeq Adapter, Index 7 (97% over 36bp)
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA1546900.427361548975512No Hit
TATATATATATATATATATATATATATATATATAAGATCGGAAGAGCACA1518960.4196425744597864No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT1504630.41568363012154924Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGC1497440.41369725121073797TruSeq Adapter, Index 7 (97% over 36bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA1429950.39505181133721207Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATATATATATATATATATATATAAGATCGGAAGAGCA1178560.3256003795724219No Hit
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG1142000.3154999605210645Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC993370.274437999809816No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCAT883910.2441975219826393TruSeq Adapter, Index 7 (97% over 36bp)
GCGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCCCGC815550.22531172749820855No Hit
CCCAGACCCGCCGCGACGCCGCCGAGAACCGCCCCCGCGCCCGCCGACAC761500.21037935195866078No Hit
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCACACG743730.20547003996351249No Hit
GGCGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCCCG722670.19965180076160918No Hit
TATATATATATATATATATATATATATATATATATATAAGATCGGAAGAG714410.19736981330635175No Hit
CGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCCCGCC688040.19008458216892576No Hit
ATATATATATATATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAG676920.1870124634640271Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATATATATATATATAGATCGGAAGAGCACA616130.17021803036413613No Hit
ATATATATATATATATATATGTAGATCGGAAGAGCACACGTCTGAACTCC599090.16551039522641378Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCAT569860.1574350328393466TruSeq Adapter, Index 7 (97% over 36bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA554180.15310312444970536No Hit
ATATATATATATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC539080.14893145246733402Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGCGGGGCCGGA523770.14470176385474426No Hit
ATATATATATATATATATATATGTAGATCGGAAGAGCACACGTCTGAACT499120.1378917165457738Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGCGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCC432520.11949215667650682No Hit
ATATATATATATATATATATATATATATATATATATAGATCGGAAGAGCA406980.11243622936327742No Hit
ATATATATATATATATATATATATGTAGATCGGAAGAGCACACGTCTGAA390780.10796066074642871Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC379980.10497694833519623No Hit
ATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCATTA377190.10420615596229449TruSeq Adapter, Index 7 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)