FastQCFastQC Report
Thu 12 May 2022
EGAF00005407973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36196518
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG8768182.422382174992633Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG8173562.2581067051808685Illumina Single End PCR Primer 1 (100% over 32bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7990752.2076018472274046No Hit
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG6501601.7961948715619551Illumina Single End PCR Primer 1 (100% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6008511.659969061112453No Hit
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT4919191.3590229866861778Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA4710831.3014594387228076Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG3804171.0509767817998406Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG3616620.9991624056214468Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT3565980.9851721096487789Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG3039890.8398294001649551Illumina Single End PCR Primer 1 (100% over 22bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG2318720.640592003904906Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGA2198200.607295983552893Illumina Single End PCR Primer 1 (97% over 36bp)
TATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGA2159230.5965297546023626Illumina Single End PCR Primer 1 (97% over 36bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGT2074210.573041307453938No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2007470.5546030698311921No Hit
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG1920040.5304488127835942No Hit
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG1613880.4458660913185075Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGT1423970.3933997187243259No Hit
TATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGACT1414190.3906978013741543Illumina Single End PCR Primer 1 (97% over 36bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGACT1330290.36751877625356116Illumina Single End PCR Primer 1 (97% over 36bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA1202610.3322446650807683Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG1053150.29095340054532315Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATATATATATATATATATAAGATCGGAAGAGCGTC1023620.282795157257944No Hit
AAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATTA952130.2630446387135912No Hit
TATATATATATATATATATATATATATATATATATAAGATCGGAAGAGCG895040.24727240338421502No Hit
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGACTGA756650.20903944407028321Illumina Single End PCR Primer 1 (97% over 36bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG737790.20382899813733463Illumina Single End PCR Primer 1 (100% over 22bp)
ACATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG605610.16731167345986153Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT584850.1615763151582702No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT540530.14933204348550874No Hit
TATATATATATATATATATATATATATATATATATATAAGATCGGAAGAG540230.14924916258519674No Hit
ACATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG525620.14521286274000167Illumina Single End PCR Primer 1 (100% over 28bp)
GGGGCGTGTTGCGTGCGGTGTGGTGGTGGGGGAGGAGGAAGGCGGGTCCG501480.13854371296156165No Hit
ACATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG492230.13598821853527457Illumina Single End PCR Primer 1 (100% over 32bp)
GTTGCGTGCGGTGTGGTGGTGGGGGAGGAGGAAGGCGGGTCCGGAAGGGG485700.13418417760514975No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA483830.1336675533265382No Hit
TATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGACTGA481620.13305699736090637Illumina Single End PCR Primer 1 (97% over 36bp)
GGCGTGTTGCGTGCGGTGTGGTGGTGGGGGAGGAGGAAGGCGGGTCCGGA479390.1324409160019204No Hit
GGGCGTGTTGCGTGCGGTGTGGTGGTGGGGGAGGAGGAAGGCGGGTCCGG438620.12117740164951778No Hit
GGGGGCGTGTTGCGTGCGGTGTGGTGGTGGGGGAGGAGGAAGGCGGGTCC438550.1211580627727783No Hit
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA423130.11689798449674083No Hit
ACATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA422310.1166714433692213Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGT415030.11466020018831646No Hit
GGGGGAGCCGCGGGGATCGCCGAGGGCCGGTCGGCCGCCCCGGGTGCCGC401330.11087530574073451No Hit
GGGAGGAGGAAGGCGGGTCCGGAAGGGGAAGGGTGCCGGCGGGGAGAGAG385720.10656273622783273No Hit
CGCGGGGATCGCCGAGGGCCGGTCGGCCGCCCCGGGTGCCGCGCGGTGCC375740.10380556494411976No Hit
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGGAGGGAAAGAG368400.1017777455831525Illumina Single End PCR Primer 1 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)