FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005407975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36511825
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA649230.17781362613345128Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG614650.16834272184422444Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG555330.1520959305649608Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG511340.14004777904144752Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA393880.10787737945172557Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATG373940.10241613504665954Illumina Single End PCR Primer 1 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)