FastQCFastQC Report
Thu 12 May 2022
EGAF00005407981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32632697
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1031940.31622884250112704Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG916520.2808594091993071Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG457000.14004358879684387Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTG440430.13496585954878323Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG433900.1329647990786664Illumina Single End PCR Primer 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)