FastQCFastQC Report
Thu 12 May 2022
EGAF00005407990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005407990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113935770
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT4187450.367527248027551No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2027010.17790813192380234No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1955850.17166250774449499No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1803680.15830673720816563No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1599680.14040191241082584No Hit
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC1412150.12394263890962424No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1411870.12391806365990242Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1292420.11343408659106793No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1268460.11133114736487058Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT1189070.10436318638123918No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)