FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14788098
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGTAT373840.25279789192633156TruSeq Adapter, Index 23 (97% over 37bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT301450.2038463634741939No Hit
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG277010.1873195592834183Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC255590.1728349379345471Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA239440.16191399326674735No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCGCGTAT223120.150878091286655TruSeq Adapter, Index 23 (97% over 37bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC211200.1428175550364895Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT193590.1309093299219413No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT189720.1282923605185738No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC179000.12104328764929743Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG177440.11998838525414154No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC173020.11699949513453319Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC168100.11367249527288768Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT159650.10795844063245996Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC151170.10222409940751002No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)