FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14788098
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG353620.2391247339583495No Hit
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG246370.16660019429138218Illumina Single End PCR Primer 1 (100% over 30bp)
ATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGC245630.1660997918731672No Hit
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG230580.15592268863784917Illumina Single End PCR Primer 1 (100% over 32bp)
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC193800.13105133601359686No Hit
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG184360.1246678240839356Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT162380.10980451982398277Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG156350.10572691633501483Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT151400.1023796298888471Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)