FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005408002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44211272
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGTAT1759860.398056857536241TruSeq Adapter, Index 23 (97% over 37bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG890460.20141017431029806Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT890200.20135136577839244No Hit
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC826580.18696137039440983Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCGCGTAT783590.1772376058304769TruSeq Adapter, Index 23 (97% over 37bp)
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA728290.1647294834674741No Hit
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC679440.15368026506905297Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT595790.13475975086172595No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT572970.129598171253702No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC569510.12881556540603492Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC548100.12397290899026836Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC542290.12265876448883896Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG540280.1222041292998763No Hit
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT514930.11647029743907843Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC448700.10148995486942787No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)