FastQCFastQC Report
Wed 11 May 2022
EGAF00005408004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30397664
Sequences flagged as poor quality0
Sequence length151
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1044650.3436612760770038No Hit
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG722640.23772879389679416Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC649820.213773005715176Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC617320.20308139467559086No Hit
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCGGGTGCCCGGGCCCCCCTCG614570.20217671989531827No Hit
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC569940.18749467064311257Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG563240.1852905539057212No Hit
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT443420.14587305129762604Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC426410.14027722656583086Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCT424450.139632440176982No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC423710.13938900041792684Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC410710.1351123560020928Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTT407500.13405635380402914No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG406590.13375698869492078No Hit
CCCGGAACCGTCTCCTCCCCCGCACGCGCCGCAGGCCGACCCCCGGAACC380040.12502276490719813No Hit
CGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGA366330.12051254991173006No Hit
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA350650.11535425880093944Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATCTCGTAT345990.11382124626418662TruSeq Adapter, Index 4 (97% over 38bp)
CGCGTCTCGGAGGGGGACGGGCCGCTCGGCGGACGGACGGACGGAATCGC338070.11121578289700156No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA329500.1083964873090248No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA321650.10581405202715578No Hit
CTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCGGGTGCCCGGGCCCC321360.10571864995941793No Hit
GTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGT319890.10523506016778131No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)