FastQCFastQC Report
Thu 12 May 2022
EGAF00005408005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30397664
Sequences flagged as poor quality0
Sequence length151
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG659630.21700022738589383Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG599630.1972618685435828Illumina Single End PCR Primer 1 (100% over 32bp)
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC513900.16905904348439407No Hit
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG512670.16865440712812668Illumina Single End PCR Primer 1 (100% over 28bp)
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG468580.15415000310550178No Hit
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC391520.128799370899027Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG387600.12750979812132934Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA387510.12748019058306587Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC376060.12371345377065818Illumina Single End PCR Primer 1 (96% over 33bp)
CGCCGCGGGGCCCCGGCGGCGGGGGCACGGTCCCCCGCGAGGGGGGCCCG363400.11954866005493053No Hit
CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGA340930.11215664466848506No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT326120.10728455976090794No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG317000.10428432921687668No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)