FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12557547
Sequences flagged as poor quality0
Sequence length151
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT429270.3418422403674858No Hit
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG335240.26696296657300983Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC298270.23752250339974834Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCGGGTGCCCGGGCCCCCCTCG283510.2257686154788033No Hit
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC263650.2099534248209463Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC251130.19998332476876254No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG228990.18235249288734495No Hit
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT203320.1619106024448883Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC195100.1553647380336303Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC193210.15385966701936293Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC193180.15383577700326345Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATCTCGTAT179450.14290211296840058TruSeq Adapter, Index 4 (97% over 38bp)
CGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCT174970.13933453723087796No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG169030.13460431404318057No Hit
GGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTT165440.13174547544994256No Hit
CCCGGAACCGTCTCCTCCCCCGCACGCGCCGCAGGCCGACCCCCGGAACC164970.1313711985310507No Hit
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA160530.12783547614832738Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGA149360.1189404268206203No Hit
CTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCGGGTGCCCGGGCCCC142920.11381203669793152No Hit
CGCGTCTCGGAGGGGGACGGGCCGCTCGGCGGACGGACGGACGGAATCGC142340.11335016305334154No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA133290.10614334152999785No Hit
GTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGT131040.10435159032253671No Hit
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG129710.10329246627545968Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA127180.10127774158440338No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)