FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12557547
Sequences flagged as poor quality0
Sequence length151
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG299110.23819142385053385Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG269400.21453234457334702Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG230530.18357884704711835Illumina Single End PCR Primer 1 (100% over 28bp)
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC209120.16652933889078814No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG192280.153119076520279No Hit
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC176810.14079979155164618Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC175070.13941417061787623Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG174990.13935046390827763Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA170470.13575103481595568Illumina Single End PCR Primer 1 (100% over 26bp)
CGCCGCGGGGCCCCGGCGGCGGGGGCACGGTCCCCCGCGAGGGGGGCCCG164280.1308217281607626No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG138490.11028427765390804No Hit
CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGA138380.11019668092820994No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT131950.105076254144221No Hit
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG128450.10228908559928145Illumina Single End PCR Primer 1 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)