FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21976386
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCAATTAGTACTGGTTAGCTGAACGCATTACTGCGCTTCCACACCCA812370.36965586607370293No Hit
GTCAGCATTCGCACTTCTGATACCTCCAGCATCCTTTACAAGACACCTTC696650.316999346480354No Hit
CGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCA627800.28567026443747395No Hit
CCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCC596080.27123659003805267No Hit
CTCGTATGACTGAAGCTTAGAGGCTTTTCTTGGAACCACTTCCAATTGCT596000.27120018732834417No Hit
GGCTTTTCTTGGAACCACTTCCAATTGCTTCGCGACCTAAAGTCGCTCGC595890.271150133602495No Hit
CCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCTCTAGAGTGCCCTTTC519590.2364310492180106No Hit
CTGGTCTCGAACGACCCTTCAGGGAGATCAAGTCTCCAGGGAATCCTCAT483630.22006803120403876No Hit
CTCTGACACACTCTAGCCGTGCAGTCACCAATGCAATTCCCAAGTTAAGC459170.2089379027106641No Hit
GTGGAATTCTACCCCCCTCTGACACACTCTAGCCGTGCAGTCACCAATGC446210.20304066373788665No Hit
CTGGGTTGTTTCCCTCTTGACACCGGACGTTAGCACCCGATGTCTGTCTC423460.19268864316453124No Hit
GGCAATTAGTACTGGTTAGCTGAACGCATTACTGCGCTTCCACACCCAGC422890.19242927385785816No Hit
GGCCGACTCACCCTACGCCGATGAACGTTGCGTAGGAAACCTTGGGCTTA420900.19152375645385916No Hit
CTCGAACGACCCTTCAGGGAGATCAAGTCTCCAGGGAATCCTCATCTTCA410940.18699161909515058No Hit
CCGGACTACGATGCATTTTCTGGGATTAGCTCCACCTCGCGGCTTGGCAA393140.17889201618500877No Hit
GCCATCTCCAATGCAGGGACCGGGACATCCAACACCCGGACAACCTTCCG378910.17241688419560888No Hit
CGCACGGGCAATTAGTACTGGTTAGCTGAACGCATTACTGCGCTTCCACA374140.17024637262923942No Hit
GTGAAGCCCTACCCATAAGGGCCATGAGGACTTGACGTCATCCCCACCTT369650.1682032705468497No Hit
GTCAACTTCACGCGTTAGCTACGTTACTAAGGAAATGAATCCCCAACAAC366240.16665160504552476No Hit
GTCGGTTTGCGGTACGGTCTCGTATGACTGAAGCTTAGAGGCTTTTCTTG364160.1657051345931037No Hit
CTCATCTTCAGGCAAGTTTCCCGCTTAGATGCTTTCAGCGGTTATCTCTT363120.16523189936689317No Hit
CCCGAGTTCTCTCAAGCGCCTTAGAATACTCATCTCGCCCACCTGTGTCG356570.16225142750950952No Hit
GGCAAGTTTCCCGCTTAGATGCTTTCAGCGGTTATCTCTTCCGTACATAG351490.1599398554430196No Hit
CGGACTACGATGCATTTTCTGGGATTAGCTCCACCTCGCGGCTTGGCAAC346850.15782849827992645No Hit
CTTCGCCATCGGTATTCCTCCACATCTCTACGCATTTCACTGCTACACGT337360.15351022684075535No Hit
CGGGGAGATTGCATCATCACAAACACTTCAACTTCGCTGAGTCTCGGGAG327900.1492056064177249No Hit
GCCGACTCACCCTACGCCGATGAACGTTGCGTAGGAAACCTTGGGCTTAC320330.14576100001155787No Hit
CTGACACACTCTAGCCGTGCAGTCACCAATGCAATTCCCAAGTTAAGCTC301080.13700159798794942No Hit
GCACCATTGTATGACGTGTGAAGCCCTACCCATAAGGGCCATGAGGACTT300540.13675587969741704No Hit
CGGGTCTACACCCAGCGACTGAACGCCCTATTCGGACTCGCTTTCGCTAC299930.1364783090358897No Hit
CTTGGTTTCGGGTCTACACCCAGCGACTGAACGCCCTATTCGGACTCGCT298270.13572295280943827No Hit
CTTTTCTTGGAACCACTTCCAATTGCTTCGCGACCTAAAGTCGCTCGCCC292070.13290174280702932No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC290650.1322555947097034Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC284500.12945713640086226Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC275330.12528447580052515Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG274680.12498870378414358Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCTCTAGAGTGCCCTTTCG267920.1219126748137751No Hit
CGCCATCGGTATTCCTCCACATCTCTACGCATTTCACTGCTACACGTGGA267450.12169880889423768No Hit
CTGAAGCTTAGAGGCTTTTCTTGGAACCACTTCCAATTGCTTCGCGACCT259690.11816774605251293No Hit
CACCATTGTATGACGTGTGAAGCCCTACCCATAAGGGCCATGAGGACTTG255860.11642496632521834No Hit
GTGGTATTTCAAGGACGGCTCCACCGAAACTAGCGTCCCGGTTTCAAAGC253500.11535108638881751No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT252700.11498705929173249No Hit
CTTACGGCGAGGGGGCCTTTCACCCCCTTTATCGCTACTCATGTCAGCAT242930.11054137836858163No Hit
CGGTCTCGTATGACTGAAGCTTAGAGGCTTTTCTTGGAACCACTTCCAAT240020.10921722980293483No Hit
CCCGCTTAGATGCTTTCAGCGGTTATCTCTTCCGTACATAGCTACCCTGC228860.10413905179859874No Hit
GTCTCGTATGACTGAAGCTTAGAGGCTTTTCTTGGAACCACTTCCAATTG225600.10265564137797725No Hit
CAAGACCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGC224100.10197309057094282No Hit
GTACAAGACCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACT222810.10138609687689322No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)