FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21976386
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATCTTGACGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGTA916690.41712499953359033No Hit
CGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTGTGCAAGA699940.3184964079171161No Hit
CGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTGTGCAAGACCGATGT646760.29429770663838906No Hit
CGGGGCTAAGCAATATACCGAAGCTGCGGATGCACGTAAGTGCATGGTAG625880.2847965994044699No Hit
CTTGACGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGTACCGCT617580.28101981827221273No Hit
CAGGCGGTTGTGCAAGACCGATGTGAAATCCCCGAGCTTAACTTGGGAAT443910.2019940858337672No Hit
CGATGCAACGCGAAAAACCTTACCTACCCTTGACATGCCACTAACGAAGC411170.18709627688556252No Hit
GGTTAAGTCCCGCAACGAGCGCAACCCTTGTCTCTAGTTGCTACGAAAGG404650.18412945604431957No Hit
CTTGGCGATTACAGGCGATGAAGGACGTAGTAGCCTGCGAAAAGCTGCGG398750.18144475620331751No Hit
ATTAATTCGATGCAACGCGAAAAACCTTACCTACCCTTGACATGCCACTA389550.1772584445868397No Hit
CTGACGCTCATGCACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTG379430.17265350180871414No Hit
CTGGGATAACACTGACGCTCATGCACGAAAGCGTGGGGAGCAAACAGGAT365390.16626482625487193No Hit
ATTCGATGCAACGCGAAAAACCTTACCTACCCTTGACATGCCACTAACGA360950.16424447586605004No Hit
GGAAGATGTAACGGGGCTAAGCAATATACCGAAGCTGCGGATGCACGTAA346270.15756457863453982No Hit
GTTGGAATCTTGACGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGAC339800.15462050948686468No Hit
CGCTGGTGTACCTGTTGTCTCGCCAGAGGCATCGCAGGGTAGCTATGTAC339220.15435658984147804No Hit
GATTAATTCGATGCAACGCGAAAAACCTTACCTACCCTTGACATGCCACT301690.13727916864947676No Hit
CCCGCAACGAGCGCAACCCTTGTCTCTAGTTGCTACGAAAGGGCACTCTA298340.13575480518043323No Hit
GCAAGACCGATGTGAAATCCCCGAGCTTAACTTGGGAATTGCATTGGTGA280570.12766885328643207No Hit
CGCCTGCCCGGTGCCGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTG273370.12439260941266685No Hit
GGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAGTCCCGGTAAACG270600.12313216558900994No Hit
AACGGGGCTAAGCAATATACCGAAGCTGCGGATGCACGTAAGTGCATGGT269930.12282729289520124No Hit
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA268880.12234950733027714Illumina Single End PCR Primer 1 (100% over 34bp)
GGCAAATTGGTACCGTAACTTCGGGATAAGGTACGCCCTTGTAGTTTGAC267190.12158050008768502No Hit
CGCAACGTTCATCGGCGTAGGGTGAGTCGGCCCCTAAGGCGAGGCAGAGA266670.12134388247457975No Hit
CACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTA263990.12012439169934494No Hit
CTCGGGAGAAGGAACTCGGCAAATTGGTACCGTAACTTCGGGATAAGGTA260310.1184498670527538No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG260050.11833155824620117Illumina Single End PCR Primer 1 (100% over 32bp)
CGCACAAGCGGTGGATGATGTGGATTAATTCGATGCAACGCGAAAAACCT259750.11819504808479428No Hit
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG254010.11558315366320922Illumina Single End PCR Primer 1 (100% over 32bp)
CGGAAAGACCCCATGAACCTTTACTGTAGCTTTGCATTGGACTTTGAACC247790.11275284298337315No Hit
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG246910.11235241317657962Illumina Single End PCR Primer 1 (100% over 30bp)
CGCAAGGCCTCATGCTATAGGAGCGGCCGATGTCTGATTAGCTAGTTGGT244480.11124668086918386No Hit
CGAAAGATTAGCTAATACCGCATACGACCTGAGGGTGAAAGTGGGGGACC242880.1105186266750138No Hit
GTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCTCTAGTTGCTACGA240770.10955850520645205No Hit
GACGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGTACCGCTGGT237920.10826165867308665No Hit
CACTGACGCTCATGCACGAAAGCGTGGGGAGCAAACAGGATTAGATACCC236940.10781572547915749No Hit
GCGGAAGGTTGTCCGGGTGTTGGATGTCCCGGTCCCTGCATTGGAGATGG235310.10707402026884676No Hit
AAGCAATATACCGAAGCTGCGGATGCACGTAAGTGCATGGTAGGAGAGCG232590.10583632813875768No Hit
GTTAAGTCCCGCAACGAGCGCAACCCTTGTCTCTAGTTGCTACGAAAGGG228960.10418455518573437No Hit
AAAGGATGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAGCGATA225460.10259193663598738No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)