FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15443896
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCAATTAGTACTGGTTAGCTGAACGCATTACTGCGCTTCCACACCCA575100.37238012998792536No Hit
GTCAGCATTCGCACTTCTGATACCTCCAGCATCCTTTACAAGACACCTTC488660.31640979711337086No Hit
CGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCA453820.29385072264148887No Hit
CCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCC436770.2828107622584353No Hit
GGCTTTTCTTGGAACCACTTCCAATTGCTTCGCGACCTAAAGTCGCTCGC427070.27652996368273913No Hit
CTCGTATGACTGAAGCTTAGAGGCTTTTCTTGGAACCACTTCCAATTGCT420240.27210750447943965No Hit
CCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCTCTAGAGTGCCCTTTC368710.2387415714273134No Hit
CTGGTCTCGAACGACCCTTCAGGGAGATCAAGTCTCCAGGGAATCCTCAT345370.22362880454517434No Hit
CTCTGACACACTCTAGCCGTGCAGTCACCAATGCAATTCCCAAGTTAAGC317450.20555046472729419No Hit
GTGGAATTCTACCCCCCTCTGACACACTCTAGCCGTGCAGTCACCAATGC308850.1999819216601821No Hit
GGCCGACTCACCCTACGCCGATGAACGTTGCGTAGGAAACCTTGGGCTTA304860.19739837667904522No Hit
CTCGAACGACCCTTCAGGGAGATCAAGTCTCCAGGGAATCCTCATCTTCA299090.1936622727840177No Hit
GGCAATTAGTACTGGTTAGCTGAACGCATTACTGCGCTTCCACACCCAGC298790.1934680212816766No Hit
CTGGGTTGTTTCCCTCTTGACACCGGACGTTAGCACCCGATGTCTGTCTC297960.1929305921251995No Hit
CCGGACTACGATGCATTTTCTGGGATTAGCTCCACCTCGCGGCTTGGCAA282940.18320506690798746No Hit
GCCATCTCCAATGCAGGGACCGGGACATCCAACACCCGGACAACCTTCCG271410.17573933416801044No Hit
CGCACGGGCAATTAGTACTGGTTAGCTGAACGCATTACTGCGCTTCCACA270410.17509182916020674No Hit
GTGAAGCCCTACCCATAAGGGCCATGAGGACTTGACGTCATCCCCACCTT259300.1678980485235073No Hit
CCCGAGTTCTCTCAAGCGCCTTAGAATACTCATCTCGCCCACCTGTGTCG257980.16704334191320636No Hit
CTCATCTTCAGGCAAGTTTCCCGCTTAGATGCTTTCAGCGGTTATCTCTT256450.16605265925126667No Hit
GTCGGTTTGCGGTACGGTCTCGTATGACTGAAGCTTAGAGGCTTTTCTTG255310.16531450354237037No Hit
CGGACTACGATGCATTTTCTGGGATTAGCTCCACCTCGCGGCTTGGCAAC253730.1642914456300405No Hit
GTCAACTTCACGCGTTAGCTACGTTACTAAGGAAATGAATCCCCAACAAC251110.16259498250959473No Hit
GGCAAGTTTCCCGCTTAGATGCTTTCAGCGGTTATCTCTTCCGTACATAG243140.157434367597399No Hit
CTTCGCCATCGGTATTCCTCCACATCTCTACGCATTTCACTGCTACACGT237730.15393136550518083No Hit
GCCGACTCACCCTACGCCGATGAACGTTGCGTAGGAAACCTTGGGCTTAC235540.15251332953809066No Hit
CGGGGAGATTGCATCATCACAAACACTTCAACTTCGCTGAGTCTCGGGAG223680.14483392014553842No Hit
GCACCATTGTATGACGTGTGAAGCCCTACCCATAAGGGCCATGAGGACTT210560.13633865444315346No Hit
CGGGTCTACACCCAGCGACTGAACGCCCTATTCGGACTCGCTTTCGCTAC210130.13606022728979786No Hit
CTTTTCTTGGAACCACTTCCAATTGCTTCGCGACCTAAAGTCGCTCGCCC209300.13552279813332077No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC209260.13549689793300862Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGACACACTCTAGCCGTGCAGTCACCAATGCAATTCCCAAGTTAAGCTC207870.13459686597216142No Hit
CTTGGTTTCGGGTCTACACCCAGCGACTGAACGCCCTATTCGGACTCGCT204230.13223994774375586No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC202820.1313269656827526Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC197910.12814771609443626Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG195950.12687860627914097Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCTCTAGAGTGCCCTTTCG188990.122371971424827No Hit
CGCCATCGGTATTCCTCCACATCTCTACGCATTTCACTGCTACACGTGGA185660.12021577974884057No Hit
CACCATTGTATGACGTGTGAAGCCCTACCCATAAGGGCCATGAGGACTTG182020.11785886152043501No Hit
CTGAAGCTTAGAGGCTTTTCTTGGAACCACTTCCAATTGCTTCGCGACCT181400.11745740841559668No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT177660.11503573968641075No Hit
CTTACGGCGAGGGGGCCTTTCACCCCCTTTATCGCTACTCATGTCAGCAT176470.1142652087271243No Hit
GTGGTATTTCAAGGACGGCTCCACCGAAACTAGCGTCCCGGTTTCAAAGC174180.11278242225925374No Hit
CGGTCTCGTATGACTGAAGCTTAGAGGCTTTTCTTGGAACCACTTCCAAT165200.10696782728917625No Hit
CCCGCTTAGATGCTTTCAGCGGTTATCTCTTCCGTACATAGCTACCCTGC161800.10476631026264356No Hit
CAAGACCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGC161070.10429363160694685No Hit
GTACAAGACCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACT160040.10362670144890901No Hit
GTCTCGTATGACTGAAGCTTAGAGGCTTTTCTTGGAACCACTTCCAATTG158570.10267486908743752No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)