FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15443896
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATCTTGACGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGTA652800.4226912690942752No Hit
CGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTGTGCAAGA501170.3245100847609955No Hit
CGGGGCTAAGCAATATACCGAAGCTGCGGATGCACGTAAGTGCATGGTAG473140.306360519392257No Hit
CGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTGTGCAAGACCGATGT465030.30110925377896874No Hit
CTTGACGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGTACCGCT441020.2855626585416012No Hit
CAGGCGGTTGTGCAAGACCGATGTGAAATCCCCGAGCTTAACTTGGGAAT314820.20384752655677038No Hit
CGATGCAACGCGAAAAACCTTACCTACCCTTGACATGCCACTAACGAAGC302390.19579903930977005No Hit
GGTTAAGTCCCGCAACGAGCGCAACCCTTGTCTCTAGTTGCTACGAAAGG297490.19262626477153175No Hit
ATTAATTCGATGCAACGCGAAAAACCTTACCTACCCTTGACATGCCACTA279150.18075102292841133No Hit
CTTGGCGATTACAGGCGATGAAGGACGTAGTAGCCTGCGAAAAGCTGCGG278570.18037547002388515No Hit
CTGACGCTCATGCACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTG267520.17322053968765394No Hit
ATTCGATGCAACGCGAAAAACCTTACCTACCCTTGACATGCCACTAACGA265780.17209388097407544No Hit
CTGGGATAACACTGACGCTCATGCACGAAAGCGTGGGGAGCAAACAGGAT257540.1667584397097727No Hit
GGAAGATGTAACGGGGCTAAGCAATATACCGAAGCTGCGGATGCACGTAA256360.1659943838005643No Hit
CGCTGGTGTACCTGTTGTCTCGCCAGAGGCATCGCAGGGTAGCTATGTAC242400.15695521389162423No Hit
GTTGGAATCTTGACGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGAC238980.1547407467649355No Hit
CCCGCAACGAGCGCAACCCTTGTCTCTAGTTGCTACGAAAGGGCACTCTA226730.14680881041933977No Hit
GATTAATTCGATGCAACGCGAAAAACCTTACCTACCCTTGACATGCCACT217580.14088413959793566No Hit
AACGGGGCTAAGCAATATACCGAAGCTGCGGATGCACGTAAGTGCATGGT201160.1302521073697984No Hit
GCAAGACCGATGTGAAATCCCCGAGCTTAACTTGGGAATTGCATTGGTGA200100.12956575206152643No Hit
CGCACAAGCGGTGGATGATGTGGATTAATTCGATGCAACGCGAAAAACCT196990.12755201148725687No Hit
CTCGGGAGAAGGAACTCGGCAAATTGGTACCGTAACTTCGGGATAAGGTA192690.1247677399537008No Hit
CACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTA191830.1242108856469896No Hit
CGCAACGTTCATCGGCGTAGGGTGAGTCGGCCCCTAAGGCGAGGCAGAGA191600.12406195949519475No Hit
GGCAAATTGGTACCGTAACTTCGGGATAAGGTACGCCCTTGTAGTTTGAC190940.12363460619004428No Hit
CGCCTGCCCGGTGCCGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTG190750.12351158023856156No Hit
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA189710.12283817503044568Illumina Single End PCR Primer 1 (100% over 34bp)
GGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAGTCCCGGTAAACG187720.12154964006491627No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG181370.11743798326536256Illumina Single End PCR Primer 1 (100% over 32bp)
CGGAAAGACCCCATGAACCTTTACTGTAGCTTTGCATTGGACTTTGAACC179060.11594224669733595No Hit
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG178170.11536596724039064Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG175310.11351410291807199Illumina Single End PCR Primer 1 (100% over 30bp)
GTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCTCTAGTTGCTACGA174830.11320330051432619No Hit
CGCAAGGCCTCATGCTATAGGAGCGGCCGATGTCTGATTAGCTAGTTGGT174200.11279537235940984No Hit
CGAAAGATTAGCTAATACCGCATACGACCTGAGGGTGAAAGTGGGGGACC173140.11210901705113788No Hit
AAGCAATATACCGAAGCTGCGGATGCACGTAAGTGCATGGTAGGAGAGCG171980.11135791124208555No Hit
GACGGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGTACCGCTGGT171290.11091113278670096No Hit
GCGGAAGGTTGTCCGGGTGTTGGATGTCCCGGTCCCTGCATTGGAGATGG170850.11062623058326732No Hit
GTTAAGTCCCGCAACGAGCGCAACCCTTGTCTCTAGTTGCTACGAAAGGG168340.10900099301367996No Hit
CACTGACGCTCATGCACGAAAGCGTGGGGAGCAAACAGGATTAGATACCC166730.10795850995111596No Hit
AAAGGATGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAGCGATA164940.10679947598714729No Hit
CGGAAGGTTGTCCGGGTGTTGGATGTCCCGGTCCCTGCATTGGAGATGGC158030.1023252163832235No Hit
CAAGCCGCGAGGTGGAGCTAATCCCAGAAAATGCATCGTAGTCCGGATCG157170.1017683620765123No Hit
GTGGACACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTG154590.10009779915637868No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)