FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14409158
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG686960.4767523543013409Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC638110.442850303952528Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC535440.3715970079584109Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTCTATCTCGTAT507030.3518803805191115TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC490510.3404154496744362Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC486150.3373895962553815Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT432660.3002673716257397Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT421320.2923973767238863No Hit
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC414410.2876018154565312Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA372020.2581830249900792Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGC363120.25200639759797205No Hit
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG354290.24587835042130846Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTCTATCGCGTAT343430.23834147699678215TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA334410.23208156923534323TruSeq Adapter, Index 7 (97% over 35bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG329380.22859073375418607Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC272410.1890533784139226Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC256900.1782893906777898No Hit
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC243380.16890646906640902No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA239860.16646357823267674No Hit
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACG234830.16297274275151957TruSeq Adapter, Index 7 (97% over 35bp)
GCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAA231530.16068253259489554No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA231220.16046739164078844No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT227840.15812166123794327Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA218620.15172295286095136Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC201960.14016086158538896No Hit
TATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA197800.1372738087818872TruSeq Adapter, Index 7 (97% over 35bp)
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG196140.13612176367279752No Hit
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCACACG177090.12290100504137717No Hit
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG173740.12057609473086492Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA156920.10890296296286014No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGT148580.1031149772943013TruSeq Adapter, Index 7 (97% over 35bp)
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT147360.10226829353942818No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG144320.10015852418302304No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)