FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14409158
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG661260.4589164752027842Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG623070.4324124976629446Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG502440.3486949063921709Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG480340.3333574383735677Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG475090.3297139222153022Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG400830.2781772536604845Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA395610.27455455759455205Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG338140.23467020071540612Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG335190.23262289163599983Illumina Single End PCR Primer 1 (100% over 24bp)
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT333750.23162352720401844No Hit
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCA327470.2272651878756552Illumina Single End PCR Primer 1 (96% over 33bp)
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG291510.2023088371992312No Hit
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG279850.19421676131249307Illumina Single End PCR Primer 1 (100% over 22bp)
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC270770.18791521336638822No Hit
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG252380.17515249676629266Illumina Single End PCR Primer 1 (100% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG243490.16898280940496316No Hit
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCAAT229310.1591418457622576Illumina Single End PCR Primer 1 (96% over 33bp)
CGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT221640.1538188421558012No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC216100.14997406510498393No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG210580.14614316811572195No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA201250.13966811940017593Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGT197070.13676718653511885No Hit
TATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCA189730.13167320394432486Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG186190.1292164330490373Illumina Single End PCR Primer 1 (100% over 24bp)
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC180450.12523285538266704No Hit
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT178310.12374768879625027No Hit
GGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGA163740.1136360639532164No Hit
CGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCG155720.10807015926954232No Hit
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCAATGG144480.10026956467546543Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)