FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20984786
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG1024710.4883109124867892Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC953110.4541909552949456Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTCTATCTCGTAT832780.39684941271261953TruSeq Adapter, Index 7 (97% over 35bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC803490.3828916816211516Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC724050.3450356844239441Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC721150.3436537308505314Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT636200.30317202186383985Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT620750.29580954506755514No Hit
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC618160.29457531756578315Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA547150.26073651644577167Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGC531570.2533120900065409No Hit
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG526310.2508055121457993Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA496690.2366905242683914TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGTCTATCGCGTAT494000.2354086431951224TruSeq Adapter, Index 7 (97% over 35bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG479790.22863707068540035Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC404420.19272057384812025Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC377760.18001613168702316No Hit
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC352210.16784064416954264No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA351310.16741176202606975No Hit
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACG343850.1638568055923944TruSeq Adapter, Index 7 (97% over 35bp)
GCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAA339420.16174575237507782No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA337180.1606783123735453No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT333920.15912480594274347Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA320650.1528011770050931Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC296570.1413261969886183No Hit
TATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA295190.14066857770195987TruSeq Adapter, Index 7 (97% over 35bp)
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG292290.13928662412854723No Hit
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG253050.120587362673129Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCACACG250610.11942461552860248No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA228340.10881216515622318No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT221270.1054430576513861No Hit
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA219600.1046472430073864No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGGT218150.10395626622068006TruSeq Adapter, Index 7 (97% over 35bp)
TATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACG213720.1018452130033635TruSeq Adapter, Index 7 (97% over 35bp)
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG213020.10151163800288455No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)