FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20984786
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG965980.460323969946608Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG917670.43730252955641297Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG740600.3529223505066957Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG697830.33254091797743374Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG694730.3310636572610271Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG593240.2827000475487336Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA560990.26733177074095493Illumina Single End PCR Primer 1 (100% over 26bp)
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT499430.23799623212740886No Hit
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG489430.23323087497771006Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCA485210.23121989426053716Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG459300.21887285388566746Illumina Single End PCR Primer 1 (100% over 24bp)
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG434270.20694516493997128No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC405720.1933400702775811No Hit
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG366810.17479806560810296Illumina Single End PCR Primer 1 (100% over 22bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG364170.17354001132058244Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCAAT336350.16028278773012028Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG335440.1598491402294977No Hit
CGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT332360.15838141022739047No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC323390.1541068848641106No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG310270.1478547362837057No Hit
TATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCA285270.13594134340945865Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA279830.13334898912002247Illumina Single End PCR Primer 1 (100% over 26bp)
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG269850.12859316268462304No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC268440.1279212473265155No Hit
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT261710.12471416196476819No Hit
TATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGT254360.12121162445973956No Hit
GGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGA249150.11872887338474644No Hit
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG246010.11723255123974102Illumina Single End PCR Primer 1 (100% over 24bp)
CGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCG230400.10979382872906114No Hit
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCAATGG215540.10271250800460868Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)