FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17991509
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT701630.38997840592470595No Hit
CGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCT331920.18448702663017316No Hit
GGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTT302940.1683794283181027No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG301700.16769021431165113No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA289430.16087033055426314No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC264320.14691374692361825Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA262710.14601888035072544No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC253020.14063300638095447Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG238460.1325402999826196Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC223520.12423638284037208Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGC211390.11749431356758347No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC207560.11536553159604344No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG198400.11027424103225583No Hit
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC189220.10517183411352544Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGT185010.10283184139807285No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)