FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005408020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56247152
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2209520.3928234446430283No Hit
CGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCT1060040.18846109755032575No Hit
GGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTT948400.1686129815070459No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG940490.16720668808262507No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA896220.1593360673621306No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC892730.15871559150230397Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC847660.15070274135835357Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG822070.1461531776755559Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA814080.14473266130878945No Hit
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC761350.13535796443524822Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGC659390.11723082441578553No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC643060.11432756630949065No Hit
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC641870.11411600004209992Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG625980.11129096811870581No Hit
GTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGT585500.10409415929183402No Hit
GCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAA574030.1020549449330341No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)