FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21144303
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGACCTATCTCGTAT1968520.9309930906684416TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGACCTATCGCGTAT1033410.48874157734118734TruSeq Adapter, Index 13 (97% over 38bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT863970.4086065168475877No Hit
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG684020.32350085032360726Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC627200.2966283636779136Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC580160.27438123640207013Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC543940.25725132675217527Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA538040.2544609770300776No Hit
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC518200.2450778349137354Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGT513200.24271313175941528No Hit
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT451260.21341918908369786Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA440650.2084012889902306Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA379010.17924922850377237No Hit
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG354390.1676054301719002Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC352600.1667588664426536Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG327860.15505831523507776Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC279470.13217271810756778Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT240840.11390302153729069Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAA230540.10903173303939127No Hit
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA226570.1071541587348611Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT223630.1057637132801209No Hit
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC221760.10487931430040516No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG212060.10029179018102419TruSeq Adapter, Index 2 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)