FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21144303
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG683820.32340626219743446Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG628930.2974465509693084Illumina Single End PCR Primer 1 (100% over 32bp)
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG588040.2781080085732786No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG583300.2758662699829831Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC545090.2577952084776689Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG513580.2428928491991436Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA442300.20918164103115622Illumina Single End PCR Primer 1 (100% over 26bp)
AAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATTA428270.20254628398013405No Hit
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG424050.20055047451788788Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC350220.16563326774119724Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG348660.16489548035704935No Hit
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG333650.15779664148778044Illumina Single End PCR Primer 1 (100% over 22bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG327400.1548407625448803Illumina Single End PCR Primer 1 (100% over 30bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT322040.15230580076344916No Hit
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA294260.13916751003804664No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG287060.1357623374958257No Hit
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG276410.13072551977712388Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA233380.11037488443104508Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG214370.10138428303832006Illumina Single End PCR Primer 1 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)