FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10363675
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGACCTATCTCGTAT1199741.1576395438876654TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGACCTATCGCGTAT570670.5506444383869622TruSeq Adapter, Index 13 (97% over 38bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT428460.41342477451290205No Hit
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA412650.3981695682274869No Hit
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG375570.3623907542449951Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC346580.33441805151165005Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC317440.3063006124757868Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC293310.28301736594403043Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC282620.27270249211790215Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA258450.24938064923880768No Hit
GTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGT250480.24169032703167553No Hit
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT246280.23763771056116678Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA239500.23109562968734546Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC194450.18762649349772162Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG188240.18163441057346935Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG175660.16949585933561212Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC151000.1457012112016249Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT132170.12753198069217725Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA118750.11458290616021827Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG117910.1137723828661165TruSeq Adapter, Index 2 (97% over 35bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC115390.11134081298381125No Hit
GCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAA114180.11017327347683133No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT108830.10501101202034992No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)