FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10363675
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG512480.4944964020967466No Hit
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG371570.3585311195111773Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG345240.3331250738758211Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG318030.30686990859902497Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC294770.284426132621874Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG279250.26945074985466066Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA237910.22956142488065284Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG223960.21610094874646302Illumina Single End PCR Primer 1 (100% over 24bp)
AAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATTA209860.20249573630975498No Hit
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC192950.1861791304725399Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG186240.1797045932065604No Hit
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG174730.16859849425999948Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG170300.16432394879229617Illumina Single End PCR Primer 1 (100% over 22bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT157650.15211785394659713No Hit
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG146720.14157140203643975Illumina Single End PCR Primer 1 (100% over 28bp)
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA141990.13700738396370013No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG140700.13576265176204388No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA122860.11854868084921613Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTC116420.11233466892776935Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG108110.10431627776826272Illumina Single End PCR Primer 1 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)