FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15365616
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG430670.2802816366099478Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA426210.2773790520341Illumina Single End PCR Primer 1 (100% over 34bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG198340.1290804091420741Illumina Single End PCR Primer 1 (100% over 30bp)
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG185720.12086726623911466No Hit
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG176000.11454145411417284Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG153740.10005456338359621Illumina Single End PCR Primer 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)