FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005408044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31882060
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1032750.32392825306771267Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1021390.32036512069797246Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTATCATCTCGTAT638030.2001219494599784TruSeq Adapter, Index 5 (97% over 38bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT562700.17649424158915702No Hit
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG481460.15101282664921903Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC424920.1332787153653183Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGT410080.12862406005132665No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTATCATCGCGTAT403110.12643787760263922TruSeq Adapter, Index 5 (97% over 38bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC374520.11747045203478068Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA360030.11292557632725111Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC350540.1099489807120368TruSeq Adapter, Index 5 (97% over 35bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT347900.10912092882329434Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG329090.10322105911600443Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)