FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005408050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64971227
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG1924600.29622343441351356No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG1722700.26514814011439247No Hit
CGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGC1660710.25560699353884764No Hit
CTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTA1627780.25053859610808954No Hit
CTCCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCGAGATCA1441960.22193824352432193No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC1431370.2203082912379044No Hit
CACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCT1418000.2182504572370166No Hit
CGCTCCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAA1329390.20461211237398977No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC1191620.18340734122198432No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC1082810.16665992778618757No Hit
CTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCA1050940.161754679498357No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG1048010.16130371064101962No Hit
CAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAG1027440.15813769378251083No Hit
CTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATG1026990.15806843235390952No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT936940.1442084509193585No Hit
CTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCC917990.14129177520381445No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC894810.13772404205941807No Hit
CTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGG875250.13471347862954783No Hit
CTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCG870630.13400239462924105No Hit
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC863570.13291575977162937No Hit
GTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAA855380.13165520177108553No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA850780.13094719605649435No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA833680.12831526176964458No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG801870.12341924833896088No Hit
GCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGC801640.12338384805323131No Hit
CTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCT798260.12286361776729258No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA783180.12054259033771982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACGAAGATCTCGTAT775820.11940978119437393TruSeq Adapter, Index 9 (97% over 37bp)
CTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGG766410.11796144776517764No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG763700.11754434005071199No Hit
CTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCC759390.11688096947899722No Hit
GTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGAC754530.11613294605010308No Hit
CTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATC729790.11232510661988884No Hit
CTTGGGCCCCCCACGAGCGGCGCCGGGGAGCGGGTCTTCCGTACGCCACA712040.10959312804728161No Hit
CCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTC708460.10904211490418675No Hit
CCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTAC689390.10610696947434901No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT686600.10567754861702089No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG680350.10471558433089159No Hit
CTCCCCGGCGCGGCGGGCGAGACGGGCCGGTGGTGCGCCCTCGGCGGACT671050.10328418147313118No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT659230.10146491461520343No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGA657100.10113707718649056No Hit
GGCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAA656380.10102625890072846No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)