FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5581329
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATTGCATCTCGTAT515240.9231493072707234TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATTGCATCGCGTAT355270.6365329834525074TruSeq Adapter, Index 22 (97% over 37bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT298550.5349084420574383No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA84360.15114679675754644No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT81360.1457717328614744No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA65570.11748097988848176No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)