FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005408054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90000583
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATTGCATCTCGTAT11668981.2965449346033682TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATTGCATCGCGTAT6481160.7201242240841929TruSeq Adapter, Index 22 (97% over 37bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT5275560.5861695362573374No Hit
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA1426870.1585400841236773No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1354470.15049569178901875No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1320880.14676349374314607No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1067230.11858034297400051No Hit
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG979180.10879707301451591Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG902190.10024268398350264No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)