FastQCFastQC Report
Sat 2 Jul 2022
EGAF00005408055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90000583
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT2150380.238929563378495No Hit
AAACAGTCCTGATAGTCCTACAGAGGTTAGATAGTAGGTGTCCAGCATTA1884320.2093675326525385No Hit
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG1741810.19353319077944195No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1636230.18180215565936944No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG1547480.17194110842593097No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC1267940.14088130962440543No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT1198890.1332091371008119No Hit
CCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTA1133340.12592585094698777No Hit
CGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCG1003530.11150261104419736No Hit
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG931310.1034782185799841Illumina Single End PCR Primer 1 (100% over 30bp)
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA925270.10280711181615346No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)